Genetically encoded Ca2+ indicators; expanded affinity range, color hue and compatibility with optogenetics
نویسندگان
چکیده
Takeharu Nagai*, Kazuki Horikawa, Kenta Saito and Tomoki Matsuda 1 Department of Biomolecular Science and Engineering, The Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan 2 PRESTO, Japan Science and Technology Agency, Tokyo, Japan 3 Division for Bioimaging, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan 4 Department of Systems Neuroscience, Centre for Brain Integration Research, Tokyo Medical and Dental University Graduate School of Medical and Dental Sciences, Bunkyo-ku, Tokyo, Japan *Correspondence: [email protected]
منابع مشابه
Genetically-Encoded Yellow Fluorescent cAMP Indicator with an Expanded Dynamic Range for Dual-Color Imaging
Cyclic AMP is a ubiquitous second messenger, which mediates many cellular responses mainly initiated by activation of cell surface receptors. Various Förster resonance energy transfer-based ratiometric cAMP indicators have been created for monitoring the spatial and temporal dynamics of cAMP at the single-cell level. However, single fluorescent protein-based cAMP indicators have been poorly dev...
متن کاملGenetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics
Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Here we describe red, single-wavelength GECIs, "RCaMPs," engineered from circular permutation of the thermostable red fluorescent protein mRuby. High-resolution crystal structures of mRuby, the red sensor RCaMP, and the recently published red GECI R-GECO1 give insight into the chromophore environments of...
متن کاملRed fluorescent genetically encoded Ca2+ indicators for use in mitochondria and endoplasmic reticulum
Ca2+ is a key intermediary in a variety of signalling pathways and undergoes dynamic changes in its cytoplasmic concentration due to release from stores within the endoplasmic reticulum (ER) and influx from the extracellular environment. In addition to regulating cytoplasmic Ca2+ signals, these responses also affect the concentration of Ca2+ within the ER and mitochondria. Single fluorescent pr...
متن کاملGenetically Encoded Calcium Indicators for Ulti-Color Neural Activity Imaging and Combination With Optogenetics
متن کامل
New Tools to Study Astrocyte Ca2+ Signal Dynamics in Brain Networks In Vivo
Sensory information processing is a fundamental operation in the brain that is based on dynamic interactions between different neuronal populations. Astrocytes, a type of glial cells, have been proposed to represent active elements of brain microcircuits that, through dynamic interactions with neurons, provide a modulatory control of neuronal network activity. Specifically, astrocytes in differ...
متن کامل